Model moving out of map
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Dear all, I am trying to refine a Cryo-EM map at a decent resolution. The model was fit into the map in chimera and refined against the same map in Real space refinement in Phenix but after the run, a significant part of the model moved out of the density. I would appreciate any suggestion/strategy to solve this issue. Thank you Shanti Pal
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Hi Shanti Pal, this kind of outcome is definitely not expected. Could you please share map and model files and then I will investigate? Thanks, Pavel On 8/3/20 16:02, Shanti Pal Gangwar wrote:
Dear all, I am trying to refine a Cryo-EM map at a decent resolution. The model was fit into the map in chimera and refined against the same map in Real space refinement in Phenix but after the run, a significant part of the model moved out of the density.
I would appreciate any suggestion/strategy to solve this issue.
Thank you Shanti Pal
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participants (2)
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Pavel Afonine
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Shanti Pal Gangwar