[phenixbb] Re Double stranded DNA restraints?

Thomas C. Terwilliger terwilliger at lanl.gov
Tue Apr 6 13:25:29 PDT 2010

Hi Sena,

I would add this command


to force autobuild to just try and build helical segments. This probably
won't fix the lack of connectivity however.

All the best,
Tom T

Hi Everyone,

Is there a way to force phenix (autobuild) into building a contiguous
double stranded DNA. Currently, when I am solving a protein-DNA complex
using SAD data:

1) its not recognizing that DNA needs to be double stranded, so it is
fitting one of the strands correctly (at least part of it), but the other
strand is in the protein density region.

2) It breaks down each of the strand. So it is not fitting as a contiguous
30 mer but instead a 10 mer, two 6 mers and a 8 mer.


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