[phenixbb] Simple Phaser Question
epozh001 at umaryland.edu
Fri Dec 24 20:54:07 PST 2010
You could search with the dimer...
On Fri, 2010-12-24 at 17:11 -0800, Joseph Noel wrote:
> Hi All,
> I am working with a protein that is a physiological dimer. On occasion
> I obtain a unit cell with one monomer in the asymmetric unit but most
> often I obtain an enantiomorphic space group with an expanded C axis
> that contains two molecules in the asymmetric unit. Anyway, that is
> all moot to some extent. I am wondering if for the case of my NCS
> dimer, is there a way using Phaser in Phenix to ensure that when both
> monomers are found, they are "close together" representative of the
> physiological dimer? When I search with individual monomers I often
> obtain a solution with two monomers but in different asymmetric units.
> Of course, I could easily do the transformation to form the
> physiological NCS dimer for later refinement but just wondering if I
> could avoid this from the get go with the appropriate Phaser keyword
> input (GUI if possible).
> Thanks and Happy Holidays!
> Joseph P. Noel, Ph.D.
> Investigator, Howard Hughes Medical Institute
> Professor, The Jack H. Skirball Center for Chemical Biology and
> The Salk Institute for Biological Studies
> 10010 North Torrey Pines Road
> La Jolla, CA 92037 USA
> Phone: (858) 453-4100 extension 1442
> Cell: (858) 349-4700
> Fax: (858) 597-0855
> E-mail: noel at salk.edu
> Web Site (Salk): http://www.salk.edu/faculty/faculty_details.php?id=37
> Web Site (HHMI): http://hhmi.org/research/investigators/noel.html
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