[phenixbb] f', f'' of Bromide?
pafonine at lbl.gov
Thu Sep 16 11:11:32 PDT 2010
once you are at the refinement stage, you can do f',f'' refinement:
On 9/16/10 11:00 AM, Tom Terwilliger wrote:
> Hi TriNgo
> I would suggest (1) see if you can ask the staff at the synchrotron
> about the f' and f" values for Br at the wavelengths you used, then if
> that fails, then (2) just enter in the AutoSol GUI the wavelengths and
> Br and hit "guess f' and f" values". This will look up values from a
> table of scattering factors. The values will be refined for you by
> AutoSol. If you have a strong anomalous signal then even if they are
> off this will work fine. However if the signal is weak then having the
> actual values can be helpful.
> All the best,
> Tom T
> On Sep 16, 2010, at 11:55 AM, Tri Ngo wrote:
>> Dear Phenix-BB members,
>> I am trying to solve the MAD data of one bacteria protein. The
>> crystal was soaked with 1M Sodium Bromide. The data were collected at
>> three different wavelength.
>> I intend to use Autosol to solve this structure, but I am not sure
>> which parameter I should put in the field: f' and f''?
>> I think there is a table in the synchrotron but maybe I didn't notice
>> about that. So now what should I do?
>> Thank you very much for your input!
>> Best wishes,
>> Structural Biology Lab
>> School of Medicine - Sungkyunkwan University
>> Phone: 031-299-6150
>> phenixbb mailing list
>> phenixbb at phenix-online.org <mailto:phenixbb at phenix-online.org>
> Thomas C. Terwilliger
> Mail Stop M888
> Los Alamos National Laboratory
> Los Alamos, NM 87545
> Tel: 505-667-0072 email: terwilliger at LANL.gov
> <mailto:terwilliger at LANL.gov>
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