[phenixbb] Subject: no kidding - we need small crystals!

Filip YABUKARSKI fyabukar at embl.fr
Thu Jul 5 12:33:19 PDT 2012


Definitely try microseeding: take a big crystal, put it in eppendorf 
with mother liquor ( the precipitant should be at a concentration higher 
than what you use to build the big crystals) and crash it by vortexing 
(put a small piece of plastic in the tube). If you use the vapor 
diffusion technique take a µl of this solution and mix it with your 
protein solution on a cover slip just as if you set a normal drop, and 
then seal the well. Normally youll get a lot of tiny crystals after day 
or two. Good luck


On 2012-07-05 01:55, jens j birktoft wrote:
> Hi everybody,
> Any suggestions regarding how to generate small!! crystals?  Yes, in
> this case that is what we want While we can generate crystals of size
> 100-500 microns, this time we something in the range of .5-5 microns.
>  Any ideas and suggestions would be greatly appreciated,
>  Cheers
>  Jens

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