Question about phenix.resolve_cryo_em
Hi, Is it possible to turn off the model refinement procedure in phenix.resolve.cryo_em and go straight into density modification? My current model fits reasonably well. Also, Does the routine work well with nucleic acid (e.g. something like RNA from ribosome)? Best wishes, Reza
Hi Reza,
If you use a model in density modification with resolve_cryo_em, it is
necessary to create a multi-model ensemble with refinement in order to
reduce model bias. For details on why this works and is necessary, see:
https://scripts.iucr.org/cgi-bin/paper?S205979832001061X
All the best,
Tom T
On Fri, Nov 17, 2023 at 4:00 PM Reza Khayat
Hi,
Is it possible to turn off the model refinement procedure in phenix.resolve.cryo_em and go straight into density modification? My current model fits reasonably well. Also, Does the routine work well with nucleic acid (e.g. something like RNA from ribosome)?
Best wishes, Reza _______________________________________________ phenixbb mailing list [email protected] http://phenix-online.org/mailman/listinfo/phenixbb Unsubscribe: [email protected]
-- Thomas C Terwilliger Laboratory Fellow, Los Alamos National Laboratory Senior Scientist, New Mexico Consortium 100 Entrada Dr, Los Alamos, NM 87544 Email: [email protected] Tel: 505-431-0010
Hi Tom,
Thanks.
Best wishes,
Reza
________________________________
From: Tom Terwilliger
participants (2)
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Reza Khayat -
Tom Terwilliger